社団法人日本物理学会 The π-electronic structure and the absorption spectra of carotenods which contain a trans-conjugated chain of 2N carbon atoms are considered within the framework of the HMO approximation. Trable 1 lists the characteristic avelength maxima and the ex-tinction alues u.sed for quantitativ-e deter-mination. RESULTS R. glutinis 48-23T, which STAHLY Department ofBacteriology, Ohio State University, Columbus Received for publication November13, 1941 The addition of a definite quantity of H2O to such solutions (ethanol, methanol, aceton, isopropanol) changes the absorption curve of these pigments in a characteristic manner. Different pigments were identified by spectrometric analysis. The absorption maxima (λ max) of carotenoids depends on the number of conjugated double bonds present in the chromophore (Britton et al ., 1995b; Davies, 1976). Beta-carotene is an antioxidant that has detoxification carotenoids exist as cis/trans isomer, however, they exist primarily in the more stable all-trans isomeric form, but cis isomers do occur. of the coloring matter. A modification of the method of Kuhn and Brockmann (Abst. Pathways and processes involved in the absorption, plasma transport, and tissue uptake of carotenoids such as J3-carotene. Specific absorption (α) coefficients for individual carotenoids and chlorophylls a and b, as well as the E 1% 1cm values for combined carotenoids, have been (re)estimated using 6 solvents (80 % acetone, chloroform, diethyl ether, dimethyl formamide, dimethyl sulphoxide, and methanol) using 2 different types of spectrophotometer (0.1—0.5 nm and 1—4 nm band pass resolution). The maxima of the xanthorhodopsin CD bands are 5-8 nm blue-shifted relative to the absorption maxima, and very nearly coincident with the maxima of the first derivative of the absorption spectrum (compare Fig. In present study, the process of II. carotenoids to vitamin A. Carotenoids are found in nearly all tissues, but, particularly, in the liver and adipose tissue. In the case of resonance regime of excitation, the difference in the amount of carbon double-bounds is negligible and overloaded by the highly dominated resonance-induced enhancement of Raman signal. .. of senescence the absorption maxima of carotenoids were ap- parent. 1); therefore we can infer that the absorbance in the blue range for yellow leaves is mainly related to carotenoids. BRIEF PAPERS accounted for approximately 25-30 per eent. The adequacy of the method is discussed. Kabocha has higher carotenoids content (285.91 mg∙100 g−1) than local pumpkins (26.62 mg∙100 g−1) [6]. THE ISOLATION AND ABSORPTION SPECTRUM MAXIMA OF BACTERIAL CAROTENOID PIGMENTS BENSOBIN AND GRANTL. Quite similar changes of reflectance have been de- scribed in the senescing leaves of a number of plant species (Adams et al., 1990; Matile et al., 1992). 316 Absorption of Light by Chlorophyll we have examined a manuscript by Zscheile and Comar (5) in which it appears that the values at the respective maxima in ether are 10 to 12 per cent higher than those However, the absorption process of this broad family of molecules is still poorly understood. Absorption maxima of carotenoids in the skin and fins of four species of marine fish (in CS2). 4). Most carotenoids exhibit absorbance maxima at wavelengths in the visible range. 2) was adapted to the extraction of carotenoid pigments from bacterial cells and to their separation. strong absorption ofretained carotenoids (Merzlyak and Gitelson, 1995; Merzylak et aL 1999). It was identified as cryptoxanthin, absorption maxima in carbon bisulphide at 513 and 482 mp. Carotenoids comprise a diverse range of naturally occurring stereoisomers, which differ in their physico-chemical properties. A preliminary account of the present paper was given in J. Chem. Phys. It is the most common and stable natural pigment in nature. Kabocha pumpkin (Cucurbita maxima) Duchesne is a potential carotenoids source. Absorption shifts in carotenoids - Influence of index of refraction and submolecular electric fields Renge, I. and Sild, Elin LU () In Journal of Photochemistry and Photobiology, A: Chemistry 218 (1). Carotenoids of each zone were eluted with acetone. Yellow leaves do not absorb at 678 run (lower curve in Fig. Most carotenoids show a 3-peak-absorption curve in the visible spectral region in polar solutions. between the absorption maximna of the isolated pigments and p)ublished data. The carotenoids are an important group of pigments in bacteria, algae and higher plants, where they function as accessory light-harvesting pigments covering regions of the visible spectrum not utilized by (bacterio)chlorophylls. II a-carotene2 which has absorption maxima at wave-lengths very close to those of a-carotene. However, phytoene and phytofluene, which 13, ). Therefore, samples of pure p-carotene were refluxed … In this study, we investigated the content changes of carotenoids in maize under three cooking methods (boiling kernels, preparing porridge and prepa 38 A general principle of photoprotection is the direct absorption of UV light using suitable compounds. absorption maxima at 420, 443, and 472 nm. 15, 15'-Dioxygenase activity responsible for vitamin A activity is widely distributed in tissues and the The molecular formula of 1 was determined to be C 40H 56O 4 by HRFABMS data. A growing literature is dedicated to the understanding of carotenoid beneficial health effects. 20 (1952) 1661. absorption maxima of these ten carotenoids were at longer wavelengths than that of flavoxanthin, the main carotenoid of calendula petals, and it is clear that these carotenoids are responsible for the orange color of the petals. Abstract— The spectroscopic (absorption and fluorescence) properties of chloroplast lamellae from wheat leaves, extracted by apolar and progressively polar solvents, show three principal characteristics: (1) When lamellae are extracted by petroleum ether at –20 C, only β‐carotene is removed; the difference (chloroplast minus residue) absorption spectrum shows a maximum at 510 nm. { Polyenes 1, 14-16, dissolved in 0.013 M2Cl2. 1. 18,19 Different carotenoids vary signifi-cantly in their absorption maxima as well as in their fine These highly lipophilic plant metabolites are usually weakly absorbed. Beta-carotene (C40H56) is one of the carotenoids and an orange-soluble fat-soluble compound. The absorption maxima of carotenoids differ from the absorption maxima of chlorophylls, thus expanding the range of light capture for photosynthesis []. Contribution from the Division of Fruit Products, University of California, Berkeley. Optical methods for noninvasive determination of carotenoids in human and animal skin Maxim E. Darvin, Martina C. Meinke, Wolfram Sterry, and Juergen Lademann Charité: Universitätsmedizin Berlin, Department of Dermatology For the estimation of physiological function of dietary carotenoids, it is essential to understand their absorption and metabolism. Absorption maxima at room temperature as function of the number of conjugated sp2 hybridised carbon atoms in polyenyl monocations. The carotenoids in spirulina were extracted, saponified, purified on the columns, characterized by absorption spectra, the behavior on the columns, absorption maxima of the reduction products and co-chromatography with authentic samples. 101, Vol. SERIAL REVIEW CAROTENOIDS 543 Figure 1. Their biosynthesis begins with phytoene, which is a rarity among carotenoids because it is colourless. The evidence for the existence of these various forms comes from derivative spectrophotometry, low etc., there are abundant β-carotene. absorbance maxima were read at 663.6 nm for Chl a, 646.6 nm for Chl b and 470.0 nm for carotenoids. The calculated values of absorption maxima are in good agreement with experiment for carotenoids. Contents of Chl a, Chl b and total carotenoids were calculated from the following equations: %ℎ H K N K Lℎ U H H = ( ä C/ I H)=12.25 # different absorption maxima, due to unique environments, e.g., Chl 660, 670, 680, 685, 690 and 700-720 nanometers (nm). z From ref.18, in 80-96% sulfuric acid. Each carotenoid shows individual features relative to its absorption, transport, and metabolism. Of 600 50 A However, in practical application, this difference could be detectable only under the excitation that is far from the absorption maxima of carotenoids (nonresonance excitation). The absorption maxima in carbon disulphide were as follows; (Fig. The presence of three secondary hydroxy groups and one tertiary hydroxy group in 1 was consistent 24 CAROTENOIDS. the light absorption maxima of the different plant constituents. Many natural foods such as green vegetables, sweet potatoes, carrots, spinach, papaya, and mango. 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